4BQL
Crystal structure of archaeal actin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-01-28 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 73.900, 88.217, 421.582 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 210.790 - 3.340 |
| R-factor | 0.20929 |
| Rwork | 0.207 |
| R-free | 0.24781 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 210.800 | 3.420 |
| High resolution limit [Å] | 3.340 | 3.340 |
| Rmerge | 0.080 | 0.300 |
| Number of reflections | 40652 | |
| <I/σ(I)> | 12 | 2.5 |
| Completeness [%] | 98.0 | 82 |
| Redundancy | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 1.2 MG/ML PROTEIN, 2.25 % POLYETHYLENEGLYCOL 6000, 0.5M NACL, 0.05 M MES PH 6. |
