4BQK
rice importin_alpha : VirD2NLS complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 300 |
Detector technology | CCD |
Collection date | 2012-01-08 |
Detector | ADSC QUANTUM 315r |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 62.803, 140.948, 72.632 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.724 - 1.997 |
R-factor | 0.1972 |
Rwork | 0.196 |
R-free | 0.22420 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4b8o |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | PHENIX ((PHENIX.REFINE: 1.8.1_1168)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.720 | 2.110 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.140 | 0.600 |
Number of reflections | 85023 | |
<I/σ(I)> | 6.1 | 2.1 |
Completeness [%] | 99.5 | 97.8 |
Redundancy | 3.8 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | 16 % PEG3350, 0.1 M BIS-TRISPROPANE PH 6.5, 0.2 M NAF, 0.2 M NDSB-221 |