4BQ1
Crystal structure of of LamAcat from Zobellia galactanivorans
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-05-17 |
Detector | ADSC QUANTUM 315r |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 44.559, 76.564, 142.119 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 71.060 - 1.500 |
R-factor | 0.139 |
Rwork | 0.138 |
R-free | 0.16640 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3iln |
RMSD bond length | 0.028 |
RMSD bond angle | 2.290 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.370 | 1.530 |
High resolution limit [Å] | 1.450 | 1.450 |
Rmerge | 0.100 | 0.460 |
Number of reflections | 86903 | |
<I/σ(I)> | 11 | 3.5 |
Completeness [%] | 99.7 | 99.7 |
Redundancy | 6.3 | 5.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 277 | MONO-CRYSTALS WERE GROWN AT 4 DEGREE CELSIUS IN HANGING DROPS COMPOSED OF 2 MICROL OF ENZYME AT 10 MG.ML-1 AND 2 MICROL OF RESERVOIR SOLUTION CONTAINING 24% PEG 3350 AND 100 MM NA CITRATE PH 5.2 |