4BPL
rice importin_alpha in complex with nucleoplasmin NLS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 300 |
| Detector technology | CCD |
| Collection date | 2012-01-08 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 62.221, 74.093, 138.027 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.890 - 2.300 |
| R-factor | 0.2014 |
| Rwork | 0.200 |
| R-free | 0.22440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4b8o |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.080 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.890 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.120 | 0.980 |
| Number of reflections | 29058 | |
| <I/σ(I)> | 19.3 | 3 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 14.5 | 14.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.2 | 0.1 M MES PH 6.2 AND 1.35 M AMMONIUM SULPHATE |
