4BPG

Crystal structure of Bacillus subtilis DltC

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	CCD
Collection date	2006-06-15
Detector	MARMOSAIC 225 mm CCD
Spacegroup name	P 61 2 2
Unit cell lengths	72.680, 72.680, 110.308
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	23.909 - 2.200
R-factor	0.2105
Rwork	0.209
R-free	0.23690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4bpf
RMSD bond length	0.002
RMSD bond angle	0.534
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	62.940	2.320
High resolution limit [Å]	2.200	2.200
Rmerge	0.100	0.810
Number of reflections	9306
<I/σ(I)>	16.6	3.8
Completeness [%]	99.9	100
Redundancy	11.2	11.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		5.7		0.1 M SODIUM ACETATE, 0.2 M AMMONIUM ACETATE, 30 % PEG 4000, PH 5.7