4BP9
Oligopeptidase B from Trypanosoma brucei with covalently bound antipain - closed form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-27 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.800, 148.800, 268.000 |
| Unit cell angles | 90.00, 91.00, 90.00 |
Refinement procedure
| Resolution | 58.590 - 2.850 |
| R-factor | 0.21609 |
| Rwork | 0.213 |
| R-free | 0.28074 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.697 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.000 | 3.000 |
| High resolution limit [Å] | 2.850 | 2.850 |
| Rmerge | 0.220 | 1.120 |
| Number of reflections | 127053 | |
| <I/σ(I)> | 8.3 | 2.2 |
| Completeness [%] | 96.7 | 97.7 |
| Redundancy | 6.5 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5 | 10 % PEG 4000, 0.2 M CALCIUM ACETATE, 0.1 M SODIUM ACETATE PH 5 |
