4BNQ

The structure of the Staphylococcus aureus Ham1 protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM30A
Synchrotron site	ESRF
Beamline	BM30A
Temperature [K]	105
Detector technology	CCD
Detector	ADSC QUANTUM 315
Spacegroup name	P 21 21 21
Unit cell lengths	54.403, 101.542, 102.016
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.398 - 2.279
R-factor	0.1908
Rwork	0.188
R-free	0.23830
Structure solution method	MAD
Starting model (for MR)	NONE
RMSD bond length	0.008
RMSD bond angle	1.137
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	autoSHARP
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.400	2.400
High resolution limit [Å]	2.280	2.280
Rmerge	0.090	0.190
Number of reflections	26528
<I/σ(I)>	5.8	3.4
Completeness [%]	85.7	85.7
Redundancy	4.1	2.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				20% AMSO4, PH 6