4BN2
The crystal structure of kinesin-like protein KIF15
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2009-11-23 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 90.080, 90.080, 249.010 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.486 - 2.695 |
| R-factor | 0.2033 |
| Rwork | 0.202 |
| R-free | 0.25910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1t5c |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.756 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((PHENIX.REFINE: 1.7.1_743)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.098 | 0.378 |
| Number of reflections | 29592 | |
| <I/σ(I)> | 8.6 | 3.1 |
| Completeness [%] | 89.7 | 86.5 |
| Redundancy | 3.7 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 292 | 25% POLYETHYLENE GLYCOL-3350 0.20 M MAGNESIUM CHLORIDE 0.1 M TRIS-HC; PH 8.5 |
