4BN0
Structure of futalosine hydrolase mutant of Helicobacter pylori strain 26695
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.230, 75.690, 97.010 |
| Unit cell angles | 90.00, 106.38, 90.00 |
Refinement procedure
| Resolution | 39.674 - 2.110 |
| R-factor | 0.212 |
| Rwork | 0.209 |
| R-free | 0.26140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4bmx |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.718 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.640 | 2.170 |
| High resolution limit [Å] | 2.110 | 2.110 |
| Rmerge | 0.130 | 0.460 |
| Number of reflections | 48788 | |
| <I/σ(I)> | 4.9 | 2.1 |
| Completeness [%] | 95.1 | 98 |
| Redundancy | 3.5 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1 M HEPES PH 7.5, 25% PEG-3350, 0.2 M NACL |
