4BMZ
Structure of futalosine hydrolase mutant of Helicobacter pylori strain 26695
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 67.445, 67.746, 100.089 |
| Unit cell angles | 90.00, 104.96, 90.00 |
Refinement procedure
| Resolution | 55.550 - 1.790 |
| R-factor | 0.18343 |
| Rwork | 0.182 |
| R-free | 0.21600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4bmx |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.843 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.480 | 1.830 |
| High resolution limit [Å] | 1.790 | 1.790 |
| Rmerge | 0.050 | 0.620 |
| Number of reflections | 40405 | |
| <I/σ(I)> | 15.8 | 2 |
| Completeness [%] | 98.4 | 99 |
| Redundancy | 4.2 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1 M BIS-TRIS PH 5.5, 25% PEG-3350, 0.2 M MGCL2.6H2O |
