4BMY
Structure of futalosine hydrolase mutant of Helicobacter pylori strain 26695
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 54.849, 123.414, 145.853 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 56.890 - 1.650 |
| R-factor | 0.17068 |
| Rwork | 0.169 |
| R-free | 0.20056 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4bmx |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.950 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.830 | 1.680 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.080 | 0.860 |
| Number of reflections | 59812 | |
| <I/σ(I)> | 16.9 | 2.1 |
| Completeness [%] | 99.9 | 99.6 |
| Redundancy | 8.2 | 7.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1 M BIS-TRIS PH 6.5, 25% PEG-3350, 0.2 M LI2SO4.H2O |
