4BMU
Crystal Structure of Ribonucleotide Reductase di-manganese(II) NrdF from Bacillus cereus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-04-15 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.880, 49.210, 98.520 |
| Unit cell angles | 90.00, 107.19, 90.00 |
Refinement procedure
| Resolution | 47.100 - 1.900 |
| R-factor | 0.1865 |
| Rwork | 0.184 |
| R-free | 0.23313 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4bmq |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.895 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.060 | 1.950 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.110 | 0.380 |
| Number of reflections | 40394 | |
| <I/σ(I)> | 11.3 | 2.1 |
| Completeness [%] | 90.4 | 92.9 |
| Redundancy | 2.1 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.2 M MG-FORMATE, 20% (W/V) PEG 3350, 0.1 M HEPES PH 7.5 |
