4BMR
Crystal Structure of Ribonucleotide Reductase apo-NrdF from Bacillus cereus (space group P21)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-02-04 |
Detector | ADSC QUANTUM 210 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 69.050, 53.850, 91.230 |
Unit cell angles | 90.00, 110.88, 90.00 |
Refinement procedure
Resolution | 34.280 - 2.000 |
R-factor | 0.21087 |
Rwork | 0.208 |
R-free | 0.25973 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4bmq |
RMSD bond length | 0.015 |
RMSD bond angle | 1.780 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.250 | 2.110 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.130 | 0.470 |
Number of reflections | 35615 | |
<I/σ(I)> | 5.7 | 2.1 |
Completeness [%] | 83.9 | 83.3 |
Redundancy | 2.3 | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.9 | 0.2 M MG-FORMATE PH 5.9 AND 20% PEG 3350 |