4BMR
Crystal Structure of Ribonucleotide Reductase apo-NrdF from Bacillus cereus (space group P21)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-04 |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.050, 53.850, 91.230 |
| Unit cell angles | 90.00, 110.88, 90.00 |
Refinement procedure
| Resolution | 34.280 - 2.000 |
| R-factor | 0.21087 |
| Rwork | 0.208 |
| R-free | 0.25973 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4bmq |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.780 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.250 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.130 | 0.470 |
| Number of reflections | 35615 | |
| <I/σ(I)> | 5.7 | 2.1 |
| Completeness [%] | 83.9 | 83.3 |
| Redundancy | 2.3 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.9 | 0.2 M MG-FORMATE PH 5.9 AND 20% PEG 3350 |
