4BMP
Crystal Structure of Bacillus cereus Ribonucleotide Reductase di- iron NrdF in Complex with NrdI (2.1 A resolution)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-02-19 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 59.330, 125.030, 142.561 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 62.590 - 2.100 |
R-factor | 0.1798 |
Rwork | 0.177 |
R-free | 0.23342 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4bmo |
RMSD bond length | 0.019 |
RMSD bond angle | 2.035 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 62.500 | 2.200 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.100 | 0.440 |
Number of reflections | 30834 | |
<I/σ(I)> | 8.6 | 2.8 |
Completeness [%] | 98.5 | 98.9 |
Redundancy | 3.9 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 10 % (W/V) PEG 8000, 0.2 M MAGNESIUM CHLORIDE AND 0.1 M TRIS, PH 7.0 |