4BMO
Crystal Structure of Bacillus cereus Ribonucleotide Reductase di- iron NrdF in Complex with NrdI (1.8 A resolution)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-12-12 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 59.490, 124.670, 141.260 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 53.750 - 1.810 |
| R-factor | 0.1689 |
| Rwork | 0.167 |
| R-free | 0.20942 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2x2o |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.903 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.700 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.060 | 0.450 |
| Number of reflections | 47107 | |
| <I/σ(I)> | 11.3 | 2.9 |
| Completeness [%] | 98.2 | 99.3 |
| Redundancy | 4.1 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 2 M SODIUM CHLORIDE AND 0.1 M SODIUM CITRATE, PH 6.0 |
