4BME
Crystal structure of the N terminal domain of human Galectin 8, F19Y mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-06-04 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 55.361, 66.645, 83.997 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.224 - 2.000 |
| R-factor | 0.1965 |
| Rwork | 0.194 |
| R-free | 0.24140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2yv8 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.036 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.220 | 1.960 |
| High resolution limit [Å] | 1.910 | 1.910 |
| Rmerge | 0.070 | 1.180 |
| Number of reflections | 24528 | |
| <I/σ(I)> | 8.6 | 1 |
| Completeness [%] | 99.1 | 94.3 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 30% W/V PEG 4000, 100 MM MES SODIUM SALT (PH 6.5) |
