4BM0
CRYSTAL STRUCTURE OF FUNGAL VERSATILE PEROXIDASE I FROM PLEUROTUS OSTREATUS - CRYSTAL FORM VII
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-11-06 |
| Detector | DECTRIS PILATUS |
| Spacegroup name | P 43 |
| Unit cell lengths | 96.700, 96.700, 38.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.350 - 2.199 |
| R-factor | 0.2301 |
| Rwork | 0.228 |
| R-free | 0.27120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3fmu |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.923 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.330 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.210 | 1.130 |
| Number of reflections | 18474 | |
| <I/σ(I)> | 11.1 | 2.1 |
| Completeness [%] | 99.3 | 96.2 |
| Redundancy | 11.3 | 10.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1 M NA-HEPES AT PH 7.5, 0.2 M NA-ACETATE & 20 % PEG 3000. |
