4BLR
P4 PROTEIN FROM BACTERIOPHAGE PHI12 IN COMPLEX WITH UTP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.2 |
| Synchrotron site | SRS |
| Beamline | PX14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 105.387, 129.330, 159.073 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.960 - 1.900 |
| R-factor | 0.19367 |
| Rwork | 0.193 |
| R-free | 0.20383 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1w44 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.007 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.070 | 0.380 |
| Number of reflections | 85262 | |
| <I/σ(I)> | 29.4 | 4.9 |
| Completeness [%] | 100.0 | 99.8 |
| Redundancy | 7.2 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.8 | 10% PEG 1500 IN 100 MM SODIUM ACETATE PH 4.8, AND 5MM AMCPP |
