4BLQ
P4 PROTEIN FROM BACTERIOPHAGE PHI8
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC QUANTUM 315r |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 138.940, 159.570, 100.330 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.790 |
R-factor | 0.215 |
Rwork | 0.214 |
R-free | 0.22920 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.010 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | BUSTER (2.11.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 72.500 | 2.850 |
High resolution limit [Å] | 2.780 | 2.780 |
Rmerge | 0.130 | 0.880 |
Number of reflections | 56752 | |
<I/σ(I)> | 14.6 | 3.3 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 11.6 | 11.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8 | 100 MM TRIS PH 8.0 AND 18% PEG 1000 |