4BLO
P4 PROTEIN FROM BACTERIOPHAGE PHI6 IN COMPLEX WITH ADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.2 |
| Synchrotron site | SRS |
| Beamline | PX14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 4r |
| Spacegroup name | P 1 |
| Unit cell lengths | 93.840, 95.260, 98.330 |
| Unit cell angles | 82.46, 86.17, 64.85 |
Refinement procedure
| Resolution | 19.990 - 2.800 |
| R-factor | 0.2183 |
| Rwork | 0.217 |
| R-free | 0.24410 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.050 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | BUSTER (2.11.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.190 | 0.860 |
| Number of reflections | 72831 | |
| <I/σ(I)> | 8.3 | 1.5 |
| Completeness [%] | 97.0 | 88.2 |
| Redundancy | 3 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 6% PEG 4000 AND 90 MM SODIUM ACETATE PH 4.5 |
