4BLK
Crystal Structure of Fungal Versatile Peroxidase I from Pleurotus ostreatus - Crystal Form I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS |
| Synchrotron site | SLS |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-11-06 |
| Detector | DECTRIS PILATUS |
| Spacegroup name | P 43 |
| Unit cell lengths | 96.500, 96.500, 38.380 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.156 - 1.049 |
| R-factor | 0.1308 |
| Rwork | 0.130 |
| R-free | 0.13690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3fmu |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.288 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.110 |
| High resolution limit [Å] | 1.050 | 1.050 |
| Rmerge | 0.070 | 0.860 |
| Number of reflections | 156095 | |
| <I/σ(I)> | 19.3 | 1.2 |
| Completeness [%] | 94.3 | 66.9 |
| Redundancy | 11 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1 M NA-ACETATE PH 4.6, 8% PEG 4000. |
