4BLG

Crystal structure of MHV-68 Latency-associated nuclear antigen (LANA) C-terminal DNA binding domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	CCD
Collection date	2012-04-29
Detector	MARRESEARCH
Spacegroup name	C 1 2 1
Unit cell lengths	88.575, 61.680, 63.678
Unit cell angles	90.00, 99.36, 90.00

Refinement procedure

Resolution	30.840 - 2.200
R-factor	0.1731
Rwork	0.171
R-free	0.20320
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1vhi
RMSD bond length	0.007
RMSD bond angle	1.168
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE: 1.8_1069))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.000	2.320
High resolution limit [Å]	2.200	2.200
Rmerge	0.060	0.640
Number of reflections	17333
<I/σ(I)>	21.4	3.3
Completeness [%]	99.9	99.9
Redundancy	7.5	7.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7		0.1 M NA/K PHOSPHATE PH 7.0, 0.1 M LITHIUM SULPHATE, 22 % W/V PEG 3350 AND 4 % V/V 1,4 DIOXANE.