4BL6
Bicaudal-D uses a parallel, homodimeric coiled coil with heterotypic registry to co-ordinate recruitment of cargos to dynein
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-03-10 |
| Detector | ADSC CCD |
| Spacegroup name | P 61 |
| Unit cell lengths | 62.199, 62.199, 190.511 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.890 - 2.180 |
| R-factor | 0.2061 |
| Rwork | 0.204 |
| R-free | 0.23518 |
| Structure solution method | MAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.027 |
| RMSD bond angle | 2.426 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.000 | 2.230 |
| High resolution limit [Å] | 2.200 | 2.180 |
| Rmerge | 0.170 | 0.170 |
| Number of reflections | 20073 | |
| <I/σ(I)> | 18 | 18 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 34 | 34.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 0.1 M TRIS PH 8.5, 5 % PEG4000, 10 % GLYCEROL, 30 MM MAGNESIUM CHLORIDE, 30 MM CALCIUM CHLORIDE |
