4BL1
Crystal structure of unphosphorylated Maternal Embryonic Leucine zipper Kinase (MELK) in complex with AMP-PNP
Replaces: 3ZGWExperimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2010-11-29 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 60.020, 63.810, 92.050 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 52.440 - 2.600 |
R-factor | 0.18354 |
Rwork | 0.180 |
R-free | 0.26024 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1zmu |
RMSD bond length | 0.025 |
RMSD bond angle | 2.677 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 63.810 | 2.740 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.130 | 0.720 |
Number of reflections | 11375 | |
<I/σ(I)> | 8.9 | 2 |
Completeness [%] | 99.9 | 99.7 |
Redundancy | 5.5 | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 10% PEG 3350, 0.1M BIS-TRIS PH 6.5, 0.2M SODIUM CHLORIDE |