4BKW
Crystal structure of the C-terminal region of human ZFYVE9
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-02-25 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 113.751, 113.751, 155.098 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.700 - 2.530 |
| R-factor | 0.2056 |
| Rwork | 0.203 |
| R-free | 0.24730 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.090 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | CCP4 |
| Refinement software | BUSTER (2.11.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.990 | 2.670 |
| High resolution limit [Å] | 2.530 | 2.530 |
| Rmerge | 0.090 | 0.560 |
| Number of reflections | 20314 | |
| <I/σ(I)> | 14.2 | 3.8 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 8.5 | 8.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.1M BIS-TRIS PH 6.5, 0.15M MAGNESIUM CHLORIDE, 30% PEG 3350 |
