4BJZ
Crystal structure of 3-hydroxybenzoate 6-hydroxylase uncovers lipid- assisted flavoprotein strategy for regioselective aromatic hydroxylation: Native data
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-03-25 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 106.790, 106.790, 142.716 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.510 |
| R-factor | 0.16984 |
| Rwork | 0.168 |
| R-free | 0.20051 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4bjy |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.928 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0033) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.700 | 1.540 |
| High resolution limit [Å] | 1.510 | 1.510 |
| Rmerge | 0.080 | 0.680 |
| Number of reflections | 63875 | |
| <I/σ(I)> | 7.6 | 1.5 |
| Completeness [%] | 99.2 | 99.4 |
| Redundancy | 3.5 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 28% PEG 4000, 0.2 M SODIUM ACETATE AND 0.1 M TRIS-HCL (PH 8.5) |
