4BJT
Crystal structure of the Rap1 C-terminal domain (Rap1-RCT) in complex with the Rap1 binding module of Rif1 (Rif1-RBM)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-06-20 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 164.010, 88.900, 57.580 |
| Unit cell angles | 90.00, 90.09, 90.00 |
Refinement procedure
| Resolution | 47.160 - 1.610 |
| R-factor | 0.1673 |
| Rwork | 0.166 |
| R-free | 0.18490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3owt |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.950 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.000 | 1.620 |
| High resolution limit [Å] | 1.610 | 1.610 |
| Rmerge | 0.040 | 0.400 |
| Number of reflections | 103829 | |
| <I/σ(I)> | 12.7 | 2.3 |
| Completeness [%] | 96.5 | 88.4 |
| Redundancy | 3.1 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 2.4-2.5 M AMMONIUM SULFATE, 100 MM NA CITRATE PH 5.0 |
