4BJS
Crystal structure of the Rif1 C-terminal domain (Rif1-CTD) from Saccharomyces cerevisiae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-03-28 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 1 |
Unit cell lengths | 34.789, 34.842, 46.214 |
Unit cell angles | 87.41, 79.89, 82.31 |
Refinement procedure
Resolution | 17.260 - 1.940 |
R-factor | 0.1721 |
Rwork | 0.171 |
R-free | 0.19630 |
Structure solution method | DIRECT METHODS |
Starting model (for MR) | 14-MER POLY-ALA IDEALIZED ALPHA-HELIX |
RMSD bond length | 0.007 |
RMSD bond angle | 0.890 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | Arcimboldo |
Refinement software | BUSTER (2.11.4) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.500 | 1.950 |
High resolution limit [Å] | 1.940 | 1.940 |
Rmerge | 0.080 | 0.360 |
Number of reflections | 15001 | |
<I/σ(I)> | 11.2 | 2.1 |
Completeness [%] | 96.4 | 73.6 |
Redundancy | 2.2 | 2.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 20% (W/V) PEG 3000, 200 MM NACL, 100 HEPES/NAOH PH 7.5. PROTEIN WAS TREATED WITH 0.003% TRYPSIN IMMEDIATELY PRIOR TO CRYSTALLIZATION. |