4BJ4
Structure of Pseudomonas aeruginosa amidase Ampdh2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF |
Synchrotron site | ESRF |
Temperature [K] | 100 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 48.759, 100.421, 104.260 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.267 - 1.722 |
R-factor | 0.1733 |
Rwork | 0.172 |
R-free | 0.20090 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2wkx |
RMSD bond length | 0.007 |
RMSD bond angle | 1.144 |
Data reduction software | XDS |
Data scaling software | SCALA |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.760 | 1.810 |
High resolution limit [Å] | 1.720 | 1.720 |
Rmerge | 0.080 | 0.560 |
Number of reflections | 54928 | |
<I/σ(I)> | 28 | 5.2 |
Completeness [%] | 99.8 | 96.5 |
Redundancy | 10.3 | 10.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.1 | 0.2 M POTASSIUM THIOCYANATE, 0.1 M BIS-TRIS PROPANE PH 7.5, 18 % PEG 3350 |