4BIH
Crystal structure of the conserved staphylococcal antigen 1A, Csa1A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.3 |
| Synchrotron site | ALS |
| Beamline | 5.0.3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-08-24 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.130, 49.250, 137.370 |
| Unit cell angles | 90.00, 89.99, 90.00 |
Refinement procedure
| Resolution | 68.685 - 2.459 |
| R-factor | 0.2094 |
| Rwork | 0.207 |
| R-free | 0.25630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4big |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.183 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 68.680 | 2.590 |
| High resolution limit [Å] | 2.460 | 2.460 |
| Rmerge | 0.100 | 0.550 |
| Number of reflections | 32851 | |
| <I/σ(I)> | 8.3 | 1.7 |
| Completeness [%] | 94.2 | 94.5 |
| Redundancy | 2.5 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.9 | 28% PEG 3350, 0.157 M NA-THIOCYANATE, PH 6.9 |
