4BHU
Crystal structure of BslA - A bacterial hydrophobin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-12-15 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 69.700, 95.980, 259.810 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 77.195 - 1.910 |
| R-factor | 0.1665 |
| Rwork | 0.165 |
| R-free | 0.19880 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.362 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHENIX (AUTOSOLVE) |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 77.190 | 1.980 |
| High resolution limit [Å] | 1.910 | 1.910 |
| Rmerge | 0.090 | 0.670 |
| Number of reflections | 135912 | |
| <I/σ(I)> | 18.04 | 4.27 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 13.3 | 13.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 1.9 M LI2SO4, 0.1 M HEPES, PH 7.5 |
