4BHQ
Structure of the periplasmic domain of the PilN type IV pilus biogenesis protein from Thermus thermophilus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | I 41 |
| Unit cell lengths | 67.830, 67.830, 147.240 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.960 - 2.050 |
| R-factor | 0.19733 |
| Rwork | 0.196 |
| R-free | 0.22623 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.682 |
| Data reduction software | XDS |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.100 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.070 | 0.570 |
| Number of reflections | 20669 | |
| <I/σ(I)> | 12.9 | 2.4 |
| Completeness [%] | 99.4 | 98.8 |
| Redundancy | 3.4 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 0.1 M HEPES PH 7.0 0.2 M MAGNESIUM CHLORIDE 20% PEG 6000 10 MM ZINC CHLORIDE |
