4BHM
The crystal structure of MoSub1-DNA complex reveals a novel DNA binding mode
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 84.190, 57.970, 83.760 |
| Unit cell angles | 90.00, 107.02, 90.00 |
Refinement procedure
| Resolution | 29.171 - 2.700 |
| R-factor | 0.187 |
| Rwork | 0.184 |
| R-free | 0.24350 |
| Structure solution method | OTHER |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.256 |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 2.700 |
| Rmerge | 0.070 |
| Number of reflections | 20278 |
| <I/σ(I)> | 6.33 |
| Completeness [%] | 94.3 |
| Redundancy | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
