4BHH
Crystal structure of tetramer of La Crosse virus nucleoprotein in complex with ssRNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-11-21 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 82.950, 86.460, 90.190 |
| Unit cell angles | 90.00, 106.63, 90.00 |
Refinement procedure
| Resolution | 44.350 - 3.400 |
| R-factor | 0.20098 |
| Rwork | 0.198 |
| R-free | 0.25213 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4bgp |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.120 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.500 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Rmerge | 0.180 | 0.790 |
| Number of reflections | 18433 | |
| <I/σ(I)> | 6.89 | 1.89 |
| Completeness [%] | 99.6 | 99.6 |
| Redundancy | 3.4 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 9 | 0.1 M BICINE PH 9 AND 10% PEG 5K MME PROTEIN:RNA RATIO 4:1.2 |
