4BH8

CRYSTAL STRUCTURE OF GERMLINE ANTIBODY 36-65 IN COMPLEX WITH PEPTIDE GDPRPSYISHLL

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	110
Detector technology	IMAGE PLATE
Collection date	2009-04-09
Detector	MARRESEARCH
Spacegroup name	P 1 21 1
Unit cell lengths	54.410, 76.970, 59.260
Unit cell angles	90.00, 101.74, 90.00

Refinement procedure

Resolution	50.000 - 2.400
R-factor	0.2305
Rwork	0.231
R-free	0.25100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2a6j
RMSD bond length	0.007
RMSD bond angle	1.693
Data reduction software	MOSFLM
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	CNS (1.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	58.000	2.500
High resolution limit [Å]	2.400	2.400
Rmerge	0.100	0.400
Number of reflections	19076
<I/σ(I)>	12	3.2
Completeness [%]	100.0	100
Redundancy	4.5	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.7		0.05 M SODIUM CACODYLATE, PH 6.7, 21% PEG