4BH7
CRYSTAL STRUCTURE OF GERMLINE ANTIBODY 36-65 IN COMPLEX WITH PEPTIDE PPYPAWHAPGNI
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-05-13 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.560, 76.770, 59.170 |
| Unit cell angles | 90.00, 102.31, 90.00 |
Refinement procedure
| Resolution | 57.810 - 2.890 |
| R-factor | 0.22993 |
| Rwork | 0.227 |
| R-free | 0.25419 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2a6j |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.804 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.000 | 3.050 |
| High resolution limit [Å] | 2.890 | 2.890 |
| Rmerge | 0.120 | 0.320 |
| Number of reflections | 10548 | |
| <I/σ(I)> | 9.4 | 3.9 |
| Completeness [%] | 97.8 | 99.7 |
| Redundancy | 4.7 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.05 M SODIUM CACODYLATE PH 6.5, 18% PEG 3000 |
