4BH2
Crystal Structure of the Haemagglutinin from a Transmissible Mutant H5 Influenza Virus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-10-10 |
| Detector | DECTRIS PILATUS 2M |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 101.620, 101.620, 331.126 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.880 - 2.120 |
| R-factor | 0.21442 |
| Rwork | 0.213 |
| R-free | 0.23517 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.095 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.990 | 2.230 |
| High resolution limit [Å] | 2.120 | 2.120 |
| Rmerge | 0.080 | 0.630 |
| Number of reflections | 36665 | |
| <I/σ(I)> | 11.5 | 2.3 |
| Completeness [%] | 97.3 | 95.6 |
| Redundancy | 4.9 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1 M HEPES PH 7.0, 25-30% JEFFAMINE ED-2001 |
