4BH1
H5 (tyTy) Influenza Virus Haemagglutinin in Complex with Avian Receptor Analogue 3'-SLN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-04-23 |
| Detector | DECTRIS PILATUS 2M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 70.859, 228.288, 71.920 |
| Unit cell angles | 90.00, 113.71, 90.00 |
Refinement procedure
| Resolution | 62.480 - 2.150 |
| R-factor | 0.20611 |
| Rwork | 0.205 |
| R-free | 0.22411 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.072 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 2.270 | 2.270 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.080 | 0.580 |
| Number of reflections | 110762 | |
| <I/σ(I)> | 9.7 | 2.1 |
| Completeness [%] | 98.1 | 94.7 |
| Redundancy | 3.5 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BIS-TRIS PROPANE PH 7.5, 0.05 - 0.15 M K/NAPO4 (PH 7.0), 15-18% PEG 3350 |
