4BGL
Superoxide reductase (Neelaredoxin) from Archaeoglobus fulgidus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-07-14 |
| Detector | MARRESEARCH |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 75.723, 75.723, 185.443 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 70.100 - 1.900 |
| R-factor | 0.18024 |
| Rwork | 0.179 |
| R-free | 0.20947 |
| Structure solution method | MAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.026 |
| RMSD bond angle | 1.951 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | HKL2Map |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.100 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.070 | 0.360 |
| Number of reflections | 42230 | |
| <I/σ(I)> | 7.2 | 2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | 200 MM AMMONIUM SULFATE, 100 MM SODIUM ACETATE PH 4.6, 15%(V/V) PEG 4000 |
