4BGJ
Crystal structure of the phox-homology domain of human sorting nexin 14
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-10-29 |
Detector | ADSC CCD |
Spacegroup name | I 41 |
Unit cell lengths | 88.611, 88.611, 73.082 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 23.490 - 2.550 |
R-factor | 0.20625 |
Rwork | 0.205 |
R-free | 0.22566 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1xtn |
RMSD bond length | 0.006 |
RMSD bond angle | 0.966 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 23.490 | 2.690 |
High resolution limit [Å] | 2.440 | 2.550 |
Rmerge | 0.050 | 1.030 |
Number of reflections | 9277 | |
<I/σ(I)> | 22.4 | 1.7 |
Completeness [%] | 99.9 | 100 |
Redundancy | 5.6 | 5.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 20%(W/V) PEG 3350, 0.2M SODIUM MALONATE |