4BGA
Nucleotide-bound open form of a putative sugar kinase MK0840 from Methanopyrus kandleri
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-06 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 73.570, 111.670, 87.880 |
| Unit cell angles | 90.00, 89.55, 90.00 |
Refinement procedure
| Resolution | 73.568 - 2.600 |
| R-factor | 0.1569 |
| Rwork | 0.154 |
| R-free | 0.20800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4BG8 CHAIN A |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.181 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 73.600 | 2.760 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.080 | 0.360 |
| Number of reflections | 54893 | |
| <I/σ(I)> | 21.95 | 5.98 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.6 | 7.69 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | MIXED WITH 5 MM ADP, 10 MM MACL2, CRYSTALLIZED IN 0.2 M POTASSIUM SODIUM TARTRATE AND 20% PEG 3350, pH 7.5 |
