4BG9
Apo form of a putative sugar kinase MK0840 from Methanopyrus kandleri (orthorhombic space group)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-02-15 |
Detector | MARMOSAIC 225 mm CCD |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 73.043, 115.967, 48.363 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 61.805 - 1.902 |
R-factor | 0.1802 |
Rwork | 0.179 |
R-free | 0.20840 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4BG8 CHAIN A |
RMSD bond length | 0.013 |
RMSD bond angle | 1.375 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 61.800 | 2.020 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.090 | 0.710 |
Number of reflections | 33085 | |
<I/σ(I)> | 14.6 | 2.17 |
Completeness [%] | 99.5 | 97.5 |
Redundancy | 4.6 | 4.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 4.6 | 293 | 0.5 M POTASSIUM THIOCYANATE, 0.1 M SODIUM ACETATE PH 4.6 AT 293 K |