4BG2
X-ray Crystal Structure of PatF from Prochloron didemni
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-12-21 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 47.080, 135.800, 48.738 |
Unit cell angles | 90.00, 118.54, 90.00 |
Refinement procedure
Resolution | 35.320 - 2.130 |
R-factor | 0.19157 |
Rwork | 0.190 |
R-free | 0.22092 |
Structure solution method | SAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.010 |
RMSD bond angle | 1.314 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHENIX (AUTOSOLVE) |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.320 | 2.190 |
High resolution limit [Å] | 2.130 | 2.130 |
Rmerge | 0.100 | 0.690 |
Number of reflections | 29355 | |
<I/σ(I)> | 14 | 3.5 |
Completeness [%] | 97.0 | 97.5 |
Redundancy | 9.5 | 9.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.4 | 0.1 M SODIUM-POTASSIUM TARTRATE, 26 % PEG 2K MME, pH 7.4 |