4BG2
X-ray Crystal Structure of PatF from Prochloron didemni
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-12-21 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.080, 135.800, 48.738 |
| Unit cell angles | 90.00, 118.54, 90.00 |
Refinement procedure
| Resolution | 35.320 - 2.130 |
| R-factor | 0.19157 |
| Rwork | 0.190 |
| R-free | 0.22092 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.314 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHENIX (AUTOSOLVE) |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.320 | 2.190 |
| High resolution limit [Å] | 2.130 | 2.130 |
| Rmerge | 0.100 | 0.690 |
| Number of reflections | 29355 | |
| <I/σ(I)> | 14 | 3.5 |
| Completeness [%] | 97.0 | 97.5 |
| Redundancy | 9.5 | 9.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.4 | 0.1 M SODIUM-POTASSIUM TARTRATE, 26 % PEG 2K MME, pH 7.4 |
