4BFO
Crystal Structure of the Starch-Binding Domain from Rhizopus oryzae Glucoamylase in Complex with isomaltotriose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13C1 |
Synchrotron site | NSRRC |
Beamline | BL13C1 |
Temperature [K] | 273 |
Detector technology | CCD |
Detector | ADSC QUANTUM 210 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 83.529, 25.866, 61.085 |
Unit cell angles | 90.00, 124.01, 90.00 |
Refinement procedure
Resolution | 25.318 - 1.175 |
R-factor | 0.1571 |
Rwork | 0.156 |
R-free | 0.16930 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2v8l |
RMSD bond length | 0.005 |
RMSD bond angle | 1.189 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.210 |
High resolution limit [Å] | 1.170 | 1.170 |
Rmerge | 0.040 | 0.170 |
Number of reflections | 36109 | |
<I/σ(I)> | 30.25 | 6.44 |
Completeness [%] | 97.8 | 86.2 |
Redundancy | 3.9 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 0.1 M NA-CITRATE PH 5.6, 18% PEG4000, 0.2 M NH4-ACETATE |