4BFL
Structure of natively expressed catalase HPII
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-12-12 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 73.402, 171.669, 123.209 |
| Unit cell angles | 90.00, 104.67, 90.00 |
Refinement procedure
| Resolution | 48.950 - 1.640 |
| R-factor | 0.1757 |
| Rwork | 0.174 |
| R-free | 0.20200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1cf9 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.010 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.950 | 1.730 |
| High resolution limit [Å] | 1.640 | 1.640 |
| Rmerge | 0.100 | 0.990 |
| Number of reflections | 290359 | |
| <I/σ(I)> | 11.1 | 1.5 |
| Completeness [%] | 81.1 | 35.4 |
| Redundancy | 5.5 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 10 % PEG 20000, 20% PEG 500MME, 30 MM SODIUM NITRATE, 30 MM DISODIUM HYDROGENPHOSPHATE, 30 MM AMMONIUM SULFATE, AND 100 MM MES/IMIDAZOLE PH 6.5 |
