4BFI
Structure of the complex of the extracellular portions of mouse CD200R and mouse CD200
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-05-21 |
| Detector | ADSC CCD |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 128.170, 128.170, 116.080 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 86.040 - 3.220 |
| R-factor | 0.20151 |
| Rwork | 0.199 |
| R-free | 0.25097 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4bfg |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.404 |
| Data reduction software | XDS (XIA2) |
| Data scaling software | XSCALE (XIA2) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 86.040 | 3.310 |
| High resolution limit [Å] | 3.220 | 3.220 |
| Rmerge | 0.120 | 0.680 |
| Number of reflections | 16034 | |
| <I/σ(I)> | 10.2 | 2.9 |
| Completeness [%] | 99.6 | 99.5 |
| Redundancy | 6.3 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 1.0M IMIDAZOLE PH7.0 |
