4BFI
Structure of the complex of the extracellular portions of mouse CD200R and mouse CD200
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-05-21 |
Detector | ADSC CCD |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 128.170, 128.170, 116.080 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 86.040 - 3.220 |
R-factor | 0.20151 |
Rwork | 0.199 |
R-free | 0.25097 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4bfg |
RMSD bond length | 0.007 |
RMSD bond angle | 1.404 |
Data reduction software | XDS (XIA2) |
Data scaling software | XSCALE (XIA2) |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 86.040 | 3.310 |
High resolution limit [Å] | 3.220 | 3.220 |
Rmerge | 0.120 | 0.680 |
Number of reflections | 16034 | |
<I/σ(I)> | 10.2 | 2.9 |
Completeness [%] | 99.6 | 99.5 |
Redundancy | 6.3 | 6.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 1.0M IMIDAZOLE PH7.0 |