4BFG
Structure of the extracellular portion of mouse CD200R
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-12-12 |
Detector | ADSC CCD |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 51.970, 51.970, 175.420 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.000 - 2.080 |
R-factor | 0.2001 |
Rwork | 0.198 |
R-free | 0.23160 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4bfe |
RMSD bond length | 0.010 |
RMSD bond angle | 1.320 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | BUSTER (2.11.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.840 | 2.130 |
High resolution limit [Å] | 2.080 | 2.080 |
Rmerge | 0.080 | 0.740 |
Number of reflections | 15423 | |
<I/σ(I)> | 16.8 | 3.1 |
Completeness [%] | 99.8 | 99.5 |
Redundancy | 7.5 | 7.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 4 | 15% PEG4000, 0.1M SODIUM ACETATE, 0.2M AMMONIUM ACETATE |