4BFC
Crystal structure of the C-terminal CMP-Kdo binding domain of WaaA from Acinetobacter baumannii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-3 |
| Synchrotron site | MAX II |
| Beamline | I911-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-02-01 |
| Detector | MARRESEARCH MAR165 |
| Spacegroup name | H 3 |
| Unit cell lengths | 110.370, 110.370, 70.340 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.330 - 1.700 |
| R-factor | 0.13518 |
| Rwork | 0.134 |
| R-free | 0.16093 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xci |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.885 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.800 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.050 | 0.460 |
| Number of reflections | 35057 | |
| <I/σ(I)> | 24.9 | 3.77 |
| Completeness [%] | 99.4 | 98.8 |
| Redundancy | 5.7 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 0.1M NA CITRATE BUFFER PH 6.0, 0.2 MM LITHIUM SULPHATE AND 17 TO 23 % PEG 3350 |
