4BEY

Night blindness causing G90D rhodopsin in complex with GaCT2 peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Detector	DECTRIS PILATUS 6M
Spacegroup name	H 3 2
Unit cell lengths	242.440, 242.440, 110.360
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.263 - 2.900
R-factor	0.2352
Rwork	0.234
R-free	0.25940
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4a4m
RMSD bond length	0.011
RMSD bond angle	1.657
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (MR)
Refinement software	PHENIX ((PHENIX.REFINE: 1.8.2_1309))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	3.070
High resolution limit [Å]	2.900	2.900
Rmerge	0.270
Number of reflections	27501
<I/σ(I)>	9.9	1.55
Completeness [%]	99.6	98.4
Redundancy	27.9	27.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1