4BEG
Structure of Rv2140c, a phosphatidyl-ethanolamine binding protein from Mycobacterium tuberculosis in complex with sulphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 62.118, 62.118, 181.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.248 - 1.420 |
| R-factor | 0.151 |
| Rwork | 0.149 |
| R-free | 0.19490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4BEF |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.017 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.010 | 1.490 |
| High resolution limit [Å] | 1.410 | 1.410 |
| Rmerge | 0.050 | 0.270 |
| Number of reflections | 67729 | |
| <I/σ(I)> | 7.86 | 2.81 |
| Completeness [%] | 97.9 | 87.2 |
| Redundancy | 5.32 | 5.23 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1M TRIS PH 8.0, 2.4 M AMMONIUM SULFATE |
